The Symbiote Saga: Part 1 is the thirteenth episode of the fourth season of Ultimate Spider-Man.
- Hydro-Man/Morris Bench (flashback)
- Mary Jane Watson
- Iron Patriot/Norman Osborn (flashback)
- Harry Osborn (flashback)
- Scarlet Spider/Ben Reilly (flashback)
- J. Jonah Jameson (cameo)
- Arnim Zola (mentioned)
- Squirrel Girl/Doreen Green (mentioned)
- Drake Bell as Spider-Man
- Tara Strong as Mary Jane Watson
- Matt Lanter as Agent Venom
- Dee Bradley Baker as Curt Connors and Carnage
- Ben Diskin as Michael Morbius
- Tom Kenny as Doctor Octopus
- Fred Tatasciore as Crossbones
- The title of this episode has the same name as the DVD of "The Symbiote Saga" from Spider-Man: The Animated Series.
- This is the first appearance of Mary Jane Watson in Season 4.