The Spider-Slayers: Part 2 is the twenty second episode of the fourth season of Ultimate Spider-Man.
- Spider-Woman/Mary Jane Watson
- Drake Bell as Spider-Man
- Tom Kenny as Doctor Octopus
- Scott Porter as Scarlet Spider
- Imari Williams as Bones-Spider and Goliath-Spider
- Roger Craig Smith as Ghost-Spider
- Tara Strong as Spider-Woman
- Matt Lanter as Agent Venom
- Eric Bauza as Iron Spider
- Ogie Banks as Kid Arachnid
- Mark Hamill as Arnim Zola
- This episode marks the first animated appearance of the white Future Foundation Spider-Man costume.