In the season premire "Hyrda Attacks", Spider-Man, Iron Spider, and Agent Venom pursue Doctor Octopus, and defeat and detain him. However, this is actually part of Ock's plan, as he has a miniture Octobot break into Swarm's cell, and bonds to his nanites, causing him to explode, sending the Octobot nanites all over the Tri-Carrier, transforming it into Hyrda Island. Doctor Octopus is reconfigured into a new life-support armor (resembling his Ends of the Earth design) and reveals that he formed an alliance with Arnim Zola and HYDRA,and has created an army of Goblin Soldiers (Goblin-mutated versions of his Spider-Soldiers). Doc Ock then releases the supervillains aboard the Tri-Carrier (Batroc the Leaper, Boomerang and Kraven the Hunter among them). He informs them he intends to reform the Sinister Six, and offers that the first one to capture Spider-Man will be the first to join. Kraven takes up this offer, and he pursues Spider-Man and his aggressive partner Scarlet Spider. Ock also intends to transform Norman Osborn back into the Green Goblin to lead his Goblin Army, but after Osborn reveals he has inoculated himself with a cure that makes him immune to the serum, he attempts to inject Harry Osborn instead. However, Spider-Man and Scarlet Spider's timely interference saves the Osborns. After the spider-related heroes manage to send Zola and Hydra Island flying into space, Doc Ock escapes and sets out to find the former members of the team to reinstate it.
Spider-Man and his Web-Warriors have been systematically eliminating various supervillains to thin out Ock's number of Sinister Six candidates.
Doctor Octopus has assembled his Sinister Six with Kraven the Hunter, Ultimate Green Goblin, Rhino, Hydro-Man, and Electro where they attack the Triskelion. After most of the Sinister Six was defeated by the S.H.I.E.L.D. Academy, Doctor Octopus revealed to Spider-Man that he actually formed the Sinister Seven when his spy on the inside is revealed to be Scarlet Spider (not Rhino) who defeats and unmasks Spider-Man.
Scarlet is ordered to retrieve the key to Dr. Connors Anti-Hydra weapon from Aunt May's house. After a fight Doc Oc was able to use to turn Hydra Island into Octopus Island. Through the efforts of Spider-Man and Doc Ock's callousness Scarlet Spider turned against him, due his respect to May for owing his debt on made him himself and learned Doc Ock only sees Scarlet as a tool, should he failed. Octopus Island was rendered incapable of flight and was going to crash into New York. Scarlet Spider gave his life to save the city.
- Doctor Octopus/Dr. Otto Octavius (Leader)
- Electro/Max Dillon
- Hydro-Man/Morris Bench
- Kraven the Hunter/Sergei Kravinoff
- Ultimate Goblin/Norman Osborn
- Rhino/Alexander Sytsevich (Blackmailed, later Unwillingly)
- Scarlet Spider/Ben Reilly (Betrayed, Defected)
Recruits For The Sinister Seven
- Boomerang/Fred Meyers
- Vulture/Adrian Toomes (Blackmailed, later Unwillingly)
- Shriek/Frances Louise Barrison
- Shocker/Herman Schultz
- Beetle/Abner Jenkins
- Grizzly/Maxwell Markham
- Green Goblin/Norman Osborn
- Batroc the Leaper/Georges Batroc
- Lizard/Dr. Curt Connors
- Anti-Venom/Harry Osborn
- Sandman/Flint Marko
Background in other media
- In the comics, The Sinister Seven consisted of Hobgoblin, Beetle, Electro, Mysterio, Scorpia, Shocker and Vulture.