May Reilly Parker
Aunt May is an old woman but is very young of nature. She has white and blue eyes, she wears red lipstick and has golden earrings. She wears a light green vest, white pants and sometimes wears a white apron while in the kitchen.
Aunt May despite her old age, is very young of nature and carefree. She has a daily schedule every day being at Monday night doing yoga, Tuesday is French cooking class, Thursday is bowling, and the rest of the days she is working. Although not being much for Peter, she cooks for him and easily can get worried about him.
Aunt May likes Peter very much, being the only family member of him now. She can be easily worried about him and would prefer to send him to another school. She doesn't know about the secret identity of Peter Parker being Spider-Man yet, until during the Contest of Champions in an unknown situation for some unknown reason.
Agent Coulson is Aunt May's current love interest. In the episode "Beetle Mania," they start dating, much to Peter's horror. However, Coulson clearly broke up with May because of constant supervillain attacks and because his relationship with her was distracting Spider-Man from his job.
During the short time that the S.H.I.E.L.D Helicarrier was destroyed, and Spider Man's team had to stay with him and Aunt May, Sam is shown to get along with her the best. They are shown cooking, weightlifting, and skateboarding together.
What she doesn't know is that secretly, Peter gained arachnid super-powers, which he used to fight crime, after the death of Ben at the hands of a Burglar.
Aunt May is involved in various hobbies such as yoga, cooking classes and bowling, which allow Peter to sneak out of the house without arousing suspicion. Aunt May was worried for Peter when he came home after Frightful Four attacked Midtown High School. She reminded Peter of the cake, which he forgot to bring home for Ben's Birthday. She didn't mind and told him that she was happy he was safe.
Back in Black
When Peter had the flu, May tries to bring him a macrobiotic shake but he is in his Spider-Man suit so he slams the door one her spilling her drink.
Back in Black
Aunt May and Peter watch the news when they learn that Spider-Man had attacked and beaten Batroc the Leaper.
Aunt May goes on a quad biking holiday leaving Peter home alone. The team watch the security tapes in the Parker house and view May reading Peter a bedtime story.
When Peter wakes up in his room he discovers he has six arms, he tries to hide it from Aunt May. But when she comes into the room she has the head of a Spider and tries to kiss Peter's forehead. At that moment, Peter woke up discovering it was just a dream.
Peter tries to sneak home after a fight with the Juggernaut, and he is able to convince Aunt May he had a good night's sleep. When Peter tries to find his chemistry set, Aunt May reveals she sold it in a yard sale.
Home Sick Hulk
The Hulk smashes back into Spider-Man's life, sick with an alien infection. Spidey has no choice but to keep the green goliath home and keep Hulk hidden from Aunt May and protect him from invading aliens.
Attack of the Beetle
Spider-Man battles the Beetle in a fancy restaurant in Manhattan while his Aunt May watches, the Beetle fires a missile at her. But the full story is revealed with a Flashback to the morning, during a meeting between Aunt May and Principal Coulson and secret S.H.I.E.L.D. handler, Agent Coulson. The two hit it off and which really annoyed Peter as his two worlds appear to be colliding. Peter is horrified when Aunt May goes on a date with his principal. Spider-Man stalks them to a restaurant and is freaked out when Coulson touches her back. Spider-Man does his best to disrupt the date by stealing food and calling him pretending to be Nick Fury. He then spills water on him and confronts Coulson in the bathroom about taking out his Aunt. Things get even more complicated when the high tech assassin, the Beetle, who is out for revenge against Coulson. The Beetle fires a missile at Aunt May but is deflected by Coulson using a tray and the patrons escape. They battle the Beetle who attacks with Missiles while Aunt May is escorted to safety by Coulson. Spider-Man threw a piano at the Beetle to no avail, he attacks and the fight leads outside. The Beetle then sets his eyes on Coulson again, but Spider-Man intervenes. The Beetle attacks Coulson who shows his impressive fighting technique. He then disrupts the armor with a fork and smoke bombs the restaurant to escape, in that time he kidnaps Aunt May. Using the Spider-Cycle they track the Beetle to the USS Excelsior. They battle the Beetle on the ship but before he can fire a missile, Aunt May escapes and uses the jet engine to blast the Beetles armor off, allowing Coulson and Spider-Man to knock him out. He was then cuffed and S.H.I.E.L.D. was called to take him away. Coulson reveals that he has only the best intentions regarding his Aunt. Coulson escorts May home, but with Peter's approval, they continue their date.
Rise of the Goblin
The S.H.I.E.L.D. Helicarrier crashes into the ocean, which is revealed to be the home of the S.H.I.E.L.D. Trainees. Peter asks Aunt May if the team could move in as they had nowhere to stay. She welcomes the team in with open arms.
Aunt May enjoys the team staying at the Parker house, but the crowded house is too much for Peter. But after battling the Lizard the team rush home for dinner.
Aunt May shows the team who are still staying at the house pictures of Peter as a child dressed as a daffodil.
Aunt May goes to look after her sick sister but decides to stay longer due to enjoying her time away.
When returns home he learns that Aunt May has gone away and the team is planning a party at the house. Due to the faulty S.H.I.E.L.D. security system the house is destroyed with Aunt May on her way. At that moment four S.H.I.E.L.D. helicopters bring a replicate house and drops it in place and after one or two minor touches everything is back to normal just in time for Aunt May's return.
The Incredible Spider-Hulk
When the Hulk wakes up in Peter's body Aunt just ignores his behavior just thinking he is acting like a teenager.
Spider-Man while fighting Deadpool remembers that if it wasn't for Aunt May or Uncle Ben he may have turned out the same.
c - cameo
f - flashback
m - mentioned
d - Dream