Anti-Venom is the eighth episode of the fourth season of Ultimate Spider-Man.
- Beetle/Abner Jenkins
- Shocker/Herman Schultz
- Shriek/Frances Barrison (cameo)
- Grizzly/Maxwell Markham (cameo)
- Nick Fury (mentioned)
- Drake Bell as Spider-Man
- Matt Lanter as Agent Venom, Harry Osborn, Anti-Venom
- Troy Baker as Shocker
- Tom Kenny as Doctor Octopus
- Mark Hamill as Arnim Zola
- Ben Diskin as Michael Morbius
- Steven Weber as Iron Patriot
- Dee Bradley Baker as Curt Connors
- This is Anti-Venom's first animated appearance.